ASSAY PROCEDURE INCUBATION TIME/ TEMP.

1. Preparation of samples

2. Incubation of samples and controls 1 hour / 23°C

3. Successive washes

4. Incubation with conjugate 30 min / 23°C

5. Successive washes

6. Revelation with substrate 10 min / 23°C

7. Stop the reaction

8. Reading optical densities 405 nm

Materials provided with the kit                                                                  Quantity                  

Components                                                                              2 plates                    5 plates

•     12 strips of 8 wells coated with M. bovis Ag	2	5
•     Ready-to-use positive control	4 mL	8 mL
•     Ready-to-use negative control •     Ready-to-use dilution buffer	4 mL 130 mL	8 mL 3 x 110 mL
•     Concentrated conjugate	125 uL	325 uL
•     Concentrated wash solution (10X)	2 x 125 mL	4 x 125 mL
•     Ready-to-use substrate	30 mL	60 mL
•     Ready-to-use stop solution	30 mL	60 mL

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