ASSAY PROCEDURE INCUBATION TIME/ TEMP.

1. Preparation of samples

2. Incubation of samples and controls 1h00 / 37°C

3. Successive washes

4. Incubation with conjugate 30 min / 37°C

5. Successive washes

6. Revelation with substrate 10 min / 23°C

7. Stop the reaction

8. Reading optical densities 450 nm

Kit components                                                                    2 plates        5 plates

	12 strips of 8 wells coated with recombinant protein S of TGEV	2	5
	Ready-to-use TGEV-Ab positive control	2 mL	6 mL
	Ready-to-use negative control	2.5 mL	6 mL
	Ready-to-use sample dilution buffer (green)	30 mL	75 mL
	Ready-to-use TGEV-Ab conjugate (blue)	11 mL	30 mL
	Concentrated wash solution (10X)*	125 mL	2 X 125 mL
	Ready-to-use substrate	25 mL	60 mL
	Ready-to-use stop solution	25 mL	60 mL